Northern Kazakhstan and Western Siberia jointly grow 16-18 Mha of spring wheat from May to September in cereal-dominated extensive rainfed production systems with average yield of 1.5-2.0 t/ha. The region is important for global food security. Wheat grain safety and nutritional value depends on its elemental composition including macro- and microelements, and toxic and trace metals. Several interrelated studies were conducted targeting ionomics analysis of spring wheat grain with focus on 23 elements: macroelements-Ca, K, Mg, P and S; microelements-B, Fe, Cu, Na, Mn and Zn; toxic elements-As, Cd, Co, Cr, Ni, Pb and Se; and trace elements-Li, Mo, Rb and Sr. The ionomics analysis was conducted at University of Nottingham in the framework of European Plant Phenotyping Network. Grain production safety study analyzed more than 200 samples collected from cropping fields across a wide area and confirmed that toxic elements were well below maximum quantities as defined by FAO, EU and national regulations (Abugalieva et al., 2021). A genotype by environment study was conducted based on multilocational trial with 49 entries at six sites in two seasons (Morgounov et al., 2022). The effect of year was least important to variation. For several elements (P, S, Cu, Mn and Mo), the effect of site was 2-3 times higher than the effect of genotype. The effects of genotype and site were similar for Ca, Mg, Fe, Cd and Sr concentrations. Broad-sense heritability was for macroelements: Mg (0.59) > Ca (0.50) > K (0.44) > P (0.30) > S (0.20); and for microelements: Zn (0.44) > Mn (0.41) > Cu (0.40) > Fe (0.38). Protein content had positive and significant genotypic correlations with Mg (0.57), P (0.60), S (0.68), Fe (0.64), Cu (0.50), Mn (0.50) and Zn (0.53). In a genetic resources study, three groups of germplasm (137 entries in total) were compared for elemental composition: primary hexaploid synthetics, cultivars from Kazakhstan and Russia, USA cultivars (Shepelev et al., 2022). Due to high variation in grain yield and protein content, element concentrations were adjusted using multiple regression analysis. Primary synthetics had significantly higher concentrations of K and Sr, compared to the local check. The synthetics from Japan had the highest concentrations of Ca, S, Cd and Mo. The US cultivars had the highest concentrations of Mg and Fe. Local germplasm had average values for most elements. Superior germplasm, with high beneficial and low toxic elements concentrations, was found in all groups of material. GWAS of this material identified several SNPs with significant effects on Ca (5A, 5D), P (1B, 6B), Mg (1B), S (4B), Mn (2B), Zn (2B, 6B), Cd (2B), Ni (3B) and Sr (5A), which were validated in multilocational trials. These studies support wheat improvement targeting optimal nutrient and metals composition.